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Targeted therapy with EDTA-NP decreased the expression of SASP Markers significantly in aortas harvested from Late-Stage CKD animals- A) SA-β Gal Activity b) transcriptional expression of IL-6, IL-1β, and MCP1 as well as activity of C) MMP 2 & 9. N=6 per group, results represented as Mean±SEM.

Journal: bioRxiv

Article Title: Targeted chelation therapy decreases NLRP3 expression by vascular cells and acts as senomorphic in Chronic Kidney Disorder induced Vascular Calcification

doi: 10.1101/2025.04.23.650230

Figure Lengend Snippet: Targeted therapy with EDTA-NP decreased the expression of SASP Markers significantly in aortas harvested from Late-Stage CKD animals- A) SA-β Gal Activity b) transcriptional expression of IL-6, IL-1β, and MCP1 as well as activity of C) MMP 2 & 9. N=6 per group, results represented as Mean±SEM.

Article Snippet: 130127, Madison, WI), EDTA (Sigma, E6511), vitamin D3 (VitD3, Cholecalciferol, Sigma, #C9756-5G), NaCl (sigma, S3014), KCl (Sigma P9541), NaHCO3 (Sigma, S5761), glucose (Sigma, G7021), NaH2PO4 (Sigma, S0876), dPBS (Gibco, 10010-031), DMEM (Gibco-1885-076), FBS, Pen-Sterp, Human Aortic Smooth Muscle Cells (SMCs) (Promocell, C12533), SMC Medium II (Promocell 22062), ABT263, HSA (Evonik Birmingham Laboratory, 777HSA097), HEPES (Sigma, H3375), absolute ethanol (Fisher BP 2818500), mPEGNHS, MW 2000 (BOC Sciences), Traut’s reagent (G-Biosciences, Saint Louis, MO, BC95), 5-Dodecanoylaminofluorescein di-β-D-Galactopyranoside (C12FDG, MedChem Express HY-126839), Cell Staining buffer (BioLegend 00-4222-26), anti-CD16/32 mouse monoclonal antibody (BioLegend Cat#156604), anti-CD45-APC (eBiosciences Cat#17-0461-82) DAPI (BioLegend Cat#422801), Rabbit anti-Rat Caspase-3 (Cell Signaling Technology, C8487), NLRP3 (Novus Biologicals, NBP2-12446), and PiT-1 (Santa Cruz Biotechnology,SC98824), anti-rabbit IgG secondary antibody Cy5 (Invitrogen, A10523) cDNA synthesis Kit (Bio-Rad, Cat# 1725034), iTaq-Universal SYBR Green Super mix 12 reagent (Bio-Rad, Cat# 172512), SA-βGal Staining Kit (Cell Signaling, 9860), Rat IL-6 ELISA Kit (RnD SYSTEMS DY-506), Rat IL-1β ELISA Kit (RnD SYSTEMS SRLB00), T-Per protein extraction buffer (Thermo Fischer, 78510), BCA assay kit (Thermo Fisher Scientific,A55865).

Techniques: Expressing, Activity Assay